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So is that a 'yes' or a 'no'? I can easily tell the difference between a Telefunkin 12ax7 tube vs. a Amperex 12ax7 tube in the amp that drives the mid-range driver of my speakers? Would I just as easily tell the difference when using the Realiser?
If your ears can "easily" tell the difference in two different tubes in an amp then there must be a significant and audible difference in the sound. I have never had such an A/B-comparison for something like this, but maybe these two tubes produce a sonic difference that others could hear as well... not just you. Or, maybe your "nose" is far more sensitive than most people's, who would not be able to tell the difference.
But if there's really an audible difference in the sound, then I would expect the very sensitive calibration microphones inserted into your ears during the PRIR processor to also be able to pick up the difference in tonal quality. Don't hold me to it of course, because this really is pure speculation without an actual test.
On the other hand, the method of calibration is to drive full-range sweep frequencies through each speaker separately, and to pick up the result in the microphones in your ears. So there may or may not be a sufficient amount of time spent on each frequency during the sweep to genuinely demonstrate the sonic difference between two tubes, which may be far more difficult to detect than the sonic difference between two speakers.
Anyway, the answer to your request for a yes/no answer is: I really haven't a clue, as I don't know that anybody has ever run this particular experiment.
But it would be very easy to set up if you wanted to. Just create two PRIR calibrations in the same listening room, one with each tube inserted in the amp. Then produce two presets... P1 and P2, with the same HPEQ and the two different PRIRs. Then listen to something you're intimately familiar with insofar as how it sounds on your system, with either tube in use. Then see if you can tell the difference and pick the right answer, doing a P1-P2 A/B-comparison.
And that would provide you with the answer you seek. Is that answer a deal-breaker, as to whether or not you buy a Realiser?
As for myself, I have far more fundamental motivations for wanting a Realiser, namely (1) the fact that I don't own a real 5.1/7.1 loudspeaker system, and (2) the ability to listen to pretty much the equivalent of the glorious zillion dollar AIX studio soundroom in my bedroom through headphones at any time day or night and as loud as I want, without bothering other people near me or the neighbors below me.