Under DSP Studio -> Output Encoding -> select DSD in DoP format (if it is 2.4), or DSDx2 in DoP format (if it is 5.6) You should not be able to choose sampling rate at all. It is greyed out. In Playback Options : Audio device -> You DSD DAC. Bitstreaming -> Either None or DSD Start playback of a DSD file. Once played back successful, select the song and right click. Select Library Tools -> Convert Format On the lower left side bar, you will see Convert Format. Select Convert file to ALAC( Or apple lossless format). Under options, leave Bitdepth as Automatic. Select Apply DSP and ensure it is the same as DSP Studio. The first part is to ensure JRiver sets itself to playback DSF properly. 2nd part is convert accordingly. On Twonky, once you are on the wireless of your HDD, go to "http://172.25.0.1:9000/webconfig". this is the Twonky config page (you need to ensure twonky is started. On the left side bar, select Advance -> Rescan Interval.