Preference is subjective, and dependant on amping, source, etc. I'm a DJ by profession, and beatmatching and low end is my stock and trade, and I felt the 400i was much better defined in this regard. Everytime I A-B'd the 500s, I came away feeling like they were 'flat' and missing something, but ultimately it's just that they weren't appealing to me. Also, the 400i's were way more comfortable, which was a big plus in my book.
I listened to the entire A-side of Coltrane's Blue Train, and selected tracks from Deadmau5' 4x4=12 LP, plus tracks from my X3. Also note I do not consider myself an audiophile, and will whole-heartedly admit to preferring a bright, warm (and therefore non-neutral) sound signature. I spent years telling myself I actually liked my old HD800s, but now consider myself old enough to say I know myself better.
On another note, I removed the fabric from the back of the screen this morning and it has transformed the cans. They've taken on some of that airiness that was the biggest thing I was missing from the 560s. Bass, if anything, seems to have firmed up and improved as well.