If I understand you correctly.
Yes. A four out interface is usually configured to output two different stereo signals (MAIN &CUE) plus allow headphone monitoring of either.
However it is a very simple matter to reconfigure them to output two stereo versions of the same input signal. I do it all the time on a MOTU Ultralite, NI Audio 2 and M-Audio Fast track Pro.You can also set them up as mono if you like. Some subs prefer that. You might have to use ASIO or supplied drivers. The better manufacturers supply exteremely versatile software. So it's easy to apply Hi Pass, Lo Pass filtersr and EQ etc.
Post up the name of your interface if you like. Check the manual.