Use a wasapi plugin that allows you to use exclusive mode. That makes this option not relevant. Most video and all popular audio software should have that.
Though in general, do select 24 bit if the dac supports 24 bit. The sampling rate option will resample everything that uses a shared renderer. If you have to resample and use a shared renderer, there are excellent plugins for that for most players. Just make sure it is the same as the windows setting so you arent resampling twice.
Edited by mindbomb - 8/16/14 at 4:24am