basically: no you shouldn't use that perf board for hi V
for a start:
Calculating Creepage and Clearance Early Avoids
bare perf board could be used safely (I still wouldn't use adjacent holes for full V diff), plan power and gnd distribution before you start wiring and you can use bus/grid or even Cu tape for low Z
you could also "air wire" Hi V nodes depending on component lead orietation - or use a classic tube era terminal strip or press in insulated standoffs for the Hi V with the rest on the perf board
double the spacing from the tables for pri/mains - sec isolation
read up - few people will be willing to say what's OK or interpret standards for you for liability reasons - including safety rated component manufacturer's - just try getting a straight answer from a transformer manufacturer
